scRNA sequencing service

Agency: HEALTH AND HUMAN SERVICES, DEPARTMENT OF
State: Maryland
Type of Government: Federal
FSC Category:
  • A - Research and development
  • B - Special Studies and Analyses - Not R&D
  • Q - Medical Services
NAICS Category:
  • 541380 - Testing Laboratories
Posted Date: Jul 29, 2025
Due Date: Aug 4, 2025
Solicitation No: NIBIB101
Original Source: Please Login to View Page
Contact information: Please Login to View Page
Bid Documents: Please Login to View Page

Description

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scRNA sequencing service
Active
Contract Opportunity
Notice ID
NIBIB101
Related Notice
Department/Ind. Agency
HEALTH AND HUMAN SERVICES, DEPARTMENT OF
Sub-tier
NATIONAL INSTITUTES OF HEALTH
Office
NATIONAL INSTITUTES OF HEALTH NINR
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General Information
  • Contract Opportunity Type: Sources Sought (Original)
  • Original Published Date: Jul 29, 2025 02:22 pm EDT
  • Original Response Date: Aug 04, 2025 08:00 am EDT
  • Inactive Policy: 15 days after response date
  • Original Inactive Date: Aug 19, 2025
  • Initiative:
    • None
Classification
  • Original Set Aside:
  • Product Service Code:
  • NAICS Code:
  • Place of Performance:
    Bethesda , MD 20892
    USA
Description

The system utilizes a microfluidics based methods of sequencing.



. The workflow includes dead cell removal to ensure high data quality.

. The sample preparation workflow includes a protocol for maximizing cell viability.

. The sequencing system has .80% of bases with quality scores of Q30 or higher for 2x150 bp sequencing with an average that is preferably .90%.

. Workflow includes barcoding cells and droplet isolation using droplet sequencing methods.

. System utilizes semiautomated processing for surface marker enrichment and cell isolation.

. Sequencing workflow includes end repair, A-tailing, ligation and PCR.

. System provides flexible sequencing depth with a capture rate > 60%.

. System capable of profiling paired V(D)J transcripts with 5fgene expression and library preparation.

. Contractor provides a robust bioinformatics pipeline for visualization and cross referencing of sequencing data.


Attachments/Links
Contact Information
Contracting Office Address
  • 6011 EXECUTIVE BLVD., SUITE 503L
  • ROCKVILLE , MD 20852
  • USA
Primary Point of Contact
Secondary Point of Contact


History
  • Jul 29, 2025 02:22 pm EDTSources Sought (Original)
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