scRNA sequencing service
| Agency: | HEALTH AND HUMAN SERVICES, DEPARTMENT OF |
|---|---|
| State: | Maryland |
| Type of Government: | Federal |
| FSC Category: |
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| NAICS Category: |
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| Posted Date: | Jul 29, 2025 |
| Due Date: | Aug 4, 2025 |
| Solicitation No: | NIBIB101 |
| Original Source: | Please Login to View Page |
| Contact information: | Please Login to View Page |
| Bid Documents: | Please Login to View Page |
Description
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- Contract Opportunity Type: Sources Sought (Original)
- Original Published Date: Jul 29, 2025 02:22 pm EDT
- Original Response Date: Aug 04, 2025 08:00 am EDT
- Inactive Policy: 15 days after response date
- Original Inactive Date: Aug 19, 2025
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Initiative:
- None
- Original Set Aside:
- Product Service Code:
- NAICS Code:
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Place of Performance:
Bethesda , MD 20892USA
The system utilizes a microfluidics based methods of sequencing.
. The workflow includes dead cell removal to ensure high data quality.
. The sample preparation workflow includes a protocol for maximizing cell viability.
. The sequencing system has .80% of bases with quality scores of Q30 or higher for 2x150 bp sequencing with an average that is preferably .90%.
. Workflow includes barcoding cells and droplet isolation using droplet sequencing methods.
. System utilizes semiautomated processing for surface marker enrichment and cell isolation.
. Sequencing workflow includes end repair, A-tailing, ligation and PCR.
. System provides flexible sequencing depth with a capture rate > 60%.
. System capable of profiling paired V(D)J transcripts with 5fgene expression and library preparation.
. Contractor provides a robust bioinformatics pipeline for visualization and cross referencing of sequencing data.
- 6011 EXECUTIVE BLVD., SUITE 503L
- ROCKVILLE , MD 20852
- USA
- Roberta Albert
- roberta.albert@nih.gov
- Jul 29, 2025 02:22 pm EDTSources Sought (Original)
See Also
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