96-Plex library prep kit

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Contract Opportunity

1. This is a combined synopsis/solicitation for commercial items prepared in accordance with the procedures contained in FAR Part 12.6, Acquisition of Commercial Items, and FAR Part 13, Simplified Acquisition Procedures, as supplemented with additional information included in this notice. This announcement constitutes the only solicitation; quotes are being requested, and a written solicitation will not be issued.

1. The solicitation number is 12405B25R0010 and is issued as a Request for Quotation (RFQ).

1. The solicitation document and incorporated provisions and clauses are those in effect through Federal Acquisition Circular (FAC) Number 2025-04.

1. This solicitation is issued as SB SET ASIDE. The associated NAICS code is 541714.

1. Description/Statement of Work/Specification: We require at least 8640 individual plant sample sequencing library kits. The kits must contain all reagents needed to prepare double stranded libraries with full length adapters compatible with Illumina sequencing chemistries from 50 nanograms of high molecular weight plant DNA. No additional primers, enzymes, or beads should be required. The kits should include unique DNA sequence barcodes for at least 960 samples that allow samples to be pooled for Illumina whole genome sequencing and later deconvoluted to associate DNA sequences with individual samples. After the step of adding one set of barcodes to individual samples, it should be possible to combine at least 96 samples within a single tube for subsequent steps. Time required to complete one set of 96 sample preparations should not exceed four hours or require equipment beyond pipettes/tips, a vortex mixer, a benchtop mini-centrifuge, 96-well thermal cycler with heated lid, a fluorometer, and Agilent Technologies 2100 Bioanalyzer.

1. Date(s) and place(s) of delivery and acceptance: 30 days after award to USDA, SEA Plant Science Research, 840 Oval Dr., NC State University, 2122 Plant Sciences, Raleigh, NC 27606

This requirement shall be FOB Destination.

1. FAR Provision 52.212-1, Instructions to Offerors-Commercial, is incorporated by reference and applies to this acquisition. Please see the attached RFQ terms and conditions for the submission requirements and its addendum.

1. Evaluation:

For the evaluation criteria, please see the attached RFQ terms and conditions.

1. Quoters are required to complete in full FAR Provision 52.212-3, Offeror Representations and Certifications-Commercial Items (DEVIATION), with their quote online at SAM: www.sam.gov. A quoter must state in their quote if they completed FAR 52.212-3 online and that is it up-to-date and valid. See the attached RFQ terms and conditions for more requirements.

System updates may lag policy updates. The System for Award Management (SAM) may continue to require entities to complete representations based on provisions that are not included in agency solicitations, including 52.223-22, Public Disclosure of Greenhouse Gas Emissions and Reduction Goals—Representation, and paragraph (t) of 52.212-3, Offeror Representations and Certifications—Commercial Products and Commercial Services. Agencies will not consider or use these representations. Entities are not required to, nor are they able to, update their entity registration to remove these representations in SAM

1. FAR Clause 52.212-4, Contract Terms and Conditions-Commercial Items, applies to this acquisition. Please see the attached RFQ terms and conditions for its Addendum.

1. FAR Clause 52.212-5, Contract Terms and Conditions Required to Implement Statutes or Executive Orders-Commercial Items (DEVIATION), is incorporated and applies to this acquisition. Please see the attached RFQ terms and conditions that are applicable to the acquisition.

1. There are no additional contract requirement(s) or terms and conditions (such as contract financing arrangements or warranty requirements) determined by the contracting officer to be necessary for this acquisition and consistent with customary commercial practices.

1. The Defense Priorities and Allocations System (DPAS) and assigned ratings are not applicable to this solicitation.

1. Date, time and place quotes are due.

Quotes are due July 25, 2025

Submit quotes to the following e-mail address(es), by the quote due date and time:

Kelly.Bero@USDA.gov

Late quotes after the established deadline may be considered only if it is in the government’s best interest and if it will not unduly delay award.

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