Genome Wide Functional Genomics CRISPR KO Screening Techniques
| Agency: | HEALTH AND HUMAN SERVICES, DEPARTMENT OF |
|---|---|
| State: | California |
| Type of Government: | Federal |
| FSC Category: |
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| NAICS Category: |
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| Set Aside: | No Set aside used |
| Posted Date: | Jul 17, 2025 |
| Due Date: | Jul 24, 2025 |
| Solicitation No: | 25-003450 |
| Original Source: | Please Login to View Page |
| Contact information: | Please Login to View Page |
| Bid Documents: | Please Login to View Page |
Description
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- Contract Opportunity Type: Combined Synopsis/Solicitation (Original)
- Original Published Date: Jul 17, 2025 10:56 am EDT
- Original Date Offers Due: Jul 24, 2025 09:00 am EDT
- Inactive Policy: 15 days after date offers due
- Original Inactive Date: Aug 08, 2025
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Initiative:
- None
- Original Set Aside: No Set aside used
- Product Service Code: Q301 - REFERENCE LABORATORY TESTING
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NAICS Code:
- 541380 - Testing Laboratories and Services
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Place of Performance:
Mountain View , CA 94043USA
The purpose of this procurement is to use Cellecta’ s Next Generation Sequencing (NGS) Services to sequence DNA samples produced as the result of CRISPR screens, for the Functional Genomics Lab. We require Cellecta to conduct NGS on the submitted samples and provide us with gene counts to determine which genes regulate the survival of wild type versus topoisomerase depleted colorectal cancer cells to elucidate mechanisms of resistance.
NCATS will provide Vendor with 15 extracted gDNA samples from our genome-wide CRISPR KO Screen and Cellecta will then amplify and sequence that DNA by NGS with an average depth of ~500 reads/guide sgRNA distributions from genomic DNA isolated from each sample and the deliverable will consist of a spreadsheet report with sgRNA read counts.
Sequencing technicians will require 6-8 weeks to amplify DNA, sequence it and for their bioinformatician to report the sequencing counts
- 9800 Medical Center Drive Bldg. B, Room #303
- Rockville , MD 20850
- USA
- Brian Lind
- brian.lind@nih.gov
- Phone Number 3015498939
- Jul 17, 2025 10:56 am EDTCombined Synopsis/Solicitation (Original)
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