NEUROHISTOLOGY: Mouse brain dissecting, embedding and staining

Agency: HEALTH AND HUMAN SERVICES, DEPARTMENT OF
State: Tennessee
Type of Government: Federal
FSC Category:
  • A - Research and development
NAICS Category:
  • 541714 - Research and Development in Biotechnology (except Nanobiotechnology)
Posted Date: Jul 2, 2020
Due Date: Jul 17, 2020
Solicitation No: HHSNNeuroSCiAss06292020
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NEUROHISTOLOGY: Mouse brain dissecting, embedding and staining
Active
Contract Opportunity
Notice ID
HHSNNeuroSCiAss06292020
Related Notice
Department/Ind. Agency
HEALTH AND HUMAN SERVICES, DEPARTMENT OF
Sub-tier
NATIONAL INSTITUTES OF HEALTH
General Information
  • Contract Opportunity Type: Special Notice (Original)
  • All Dates/Times are: (UTC-04:00) EASTERN STANDARD TIME, NEW YORK, USA
  • Original Published Date: Jul 02, 2020 11:51 am EDT
  • Original Response Date: Jul 17, 2020 11:00 am EDT
  • Inactive Policy: 15 days after response date
  • Original Inactive Date: Aug 01, 2020
  • Initiative:
    • None
Classification
  • Original Set Aside: Total Small Business Set-Aside (FAR 19.5)
  • Product Service Code:
  • NAICS Code: 541714 - Research and Development in Biotechnology (except Nanobiotechnology)
  • Place of Performance:
    TN 37934
    USA
Description

Laboratory mice are common experimental animals in biological, medical, pharmacological and psychological researches primarily because they are easy to maintain and reproduce quickly Dissection of brain tissue is an important step in sample preparation for (subcellular) proteomics studies. Some Labs (vendors) can perform brain removal and separate dissection of multiple brain regions from a single brain and follow a step-by-step protocol. This concerns dissection from fresh or frozen tissue of cerebellum, hippocampus, prefrontal cortex, and striatum for protein immunoblot and/or neuronal culture, providing technical reference information for minimizing the number of animals used in projects, and refining methods and procedures to quick brain dissection, embedding and staining. NeuroScience Associates is one of the rare companies that can perform all stages in one Lab over a period of time with consistent and accurate results.

The Lab needs to investigate the effect of overexpression of carboxypeptidase E-neurotrophic factor 1 in the hippocampus on prevention or treatment of neurodegeneration and cognitive dysfunction in the Alzheimer's Disease mouse model”, and proposes to inject AAV-CPE into mouse brain at 2 month of age and evaluate the pathophysiological and behavioral changes at 6-7 months of age, to study whether CPE has prevented the development of AD. Also, they will inject AAV-CPE into mouse brain at 4 months of age when there is intracellular accumulation of Aβ, and pathophysiological and behavioral changes evaluated at 6 -7 months of age, to determine whether CPE has any therapeutic effect.

  1. the end of our treatment of mice, the Lab will provide 50 mouse brains to the company from the experiments. By using MultiBrain® technology, 25 mouse brains will be embedded together into a single block, freeze-sectioned at 25μ in the coronal plane through the whole mouse brain. All sections that are cut will be collected into a series of 24 cups containing antigen preserve solution. Thionine Nissl stain and several antibody stains will be performed through the cortex/cerebrum (hippocampus and pre-frontal cortex) on every 12th section spaced at 300μ intervals, yielding ~30 2 x 3 slides (~30 stained sections per mouse brain, ~750 stained sections total) per stain. Approximately 30 days after the shipment of the stained slides, unstained sections for each block will be sent to NIH in antigen preserve solution, the total number of unstained sections to be sent is ~13500. These services have been provided in the past by one main vendor who can do dissecting, embedding and staining all in one Lab without the need to sub-contract one of the services…

This proposed procurement is for a service for which the government intends to solicit and negotiate on a sole source basis to NeuroScience Associates located in Knoxville, TN. 37934. In order to comply with the requirements in this Special Notice, the responses should be based on our interpretation of the general requirements provided below.


Note 22. The proposed contract action is for a continuation of previous services for which the Government intends to solicit and negotiate with only one source under the authority of FAR 6.302. Interested persons may identify their interest and capability to respond to the requirement or submit quotations. This notice of intent is not a request for competitive quotes. However, all quotes received within the time and date of publication of this "Special Note 22" will be considered by the Government. A determination by the Government not to compete with this proposed contract based upon responses to this notice is solely within the discretion of the Government. Information received will normally be considered solely for the purpose of determining whether to conduct a competitive procurement.

HOW TO RESPOND: In order to compete for this project, interested parties must be registered in the Central Contracting Registration and submit a price quotation by 11:00 am, ET, July 17th 2020. Offerors may either submit one copy of the quote to MOE FILALI by mail or preferably via email. Electronic transmissions to Moe.Filali@nih.gov are preferable but must be timely. It is the responsibility of the Offeror to ensure the proposal is received by the date and time shown above.

The following Federal Acquisition Regulations clauses are incorporated by reference: FAR 52.212-3, Offeror Representations and Certifications-Commercial Items (Mar 2005), 52.212-4 (Sept 2005), 52.212-5, Contract Terms and Conditions Required to Implement Statutes of Executive Orders-Commercial Items (Aug 2006). (6)(i) 52.222-26, Equal Opportunity (Apr 2002) (E.O. 11246). (18) 52.222-35, Equal Opportunity for Special Disabled Veterans, Veterans of the Vietnam Era, and Other Eligible Veterans (Dec 2001) (38 U.S.C. 4212). (19) 52.222-36, Affirmative Action for Workers with Disabilities (Jun 1998) (29 U.S.C. 793). (20) 52.222-37, Employment Reports on Special Disabled Veterans, Veterans of the Vietnam Era, and Other Eligible Veterans (Dec 2001) (38 U.S.C. 4212). (31) 52.232-33, Payment by Electronic Funds Transfer-Central Contractor Registration (Oct 2003) (31 U.S.C. 3332).

Primary Point of Contact:

MOE FILALI Contracting Office Address:

NIH/NICHD Office of Acquisitions

6710B Rockledge Drive, Room 1145D

Bethesda, MD 20817

(w) 301.827 7737

Moe.filali@nih.gov


Attachments/Links
Contact Information
Primary Point of Contact


Secondary Point of Contact


History
  • Jul 02, 2020 11:51 am EDTSpecial Notice (Original)

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